Immersion Oil and Refractive Index

Objective
Front Lens

n = 1.00

Object
Space

Cover Glass

Microscope Slide

Specimen

Key

NA - Numerical Aperture

θ - Angular Aperture

n - Refractive Index

Formula

NA = n sin(θ)

0.90 = 1.00 sin(65°)

Refractive Index (n)

Low

High